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eescs  (Dojindo Labs)


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    Dojindo Labs eescs
    Figure 3. The effects of IGF2BP1 silencing on <t>eESCs.</t> A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The <t>cell</t> <t>viability</t> of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.
    Eescs, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 57214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eescs/product/Dojindo Labs
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    1) Product Images from "Inhibition of IGF2BP1 attenuates the progression of endometriosis through PTBP1."

    Article Title: Inhibition of IGF2BP1 attenuates the progression of endometriosis through PTBP1.

    Journal: Folia histochemica et cytobiologica

    doi: 10.5603/fhc.98213

    Figure 3. The effects of IGF2BP1 silencing on eESCs. A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The cell viability of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.
    Figure Legend Snippet: Figure 3. The effects of IGF2BP1 silencing on eESCs. A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The cell viability of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.

    Techniques Used: Inverted Microscopy, Flow Cytometry, Migration

    Figure 4. The effects of IGF2BP1/PTBP1 on eESCs. A. The mRNA levels of IGF2BP1 and PTBP1 in eESCs. B. The cell viability of eESCs was evaluated. C. The proliferation was detected using an EDU assay. D. eESC migration and invasion were detected using Transwell assay. E. Flow cytometry was conducted to identify the apoptotic eESC population. F. The expression levels of PCNA, VEGF, and E-cadherin in eESCs were assessed as described in Methods. n = 3 per group. *P < 0.05 vs. si-IGF2BP1+ +oe-NC group.
    Figure Legend Snippet: Figure 4. The effects of IGF2BP1/PTBP1 on eESCs. A. The mRNA levels of IGF2BP1 and PTBP1 in eESCs. B. The cell viability of eESCs was evaluated. C. The proliferation was detected using an EDU assay. D. eESC migration and invasion were detected using Transwell assay. E. Flow cytometry was conducted to identify the apoptotic eESC population. F. The expression levels of PCNA, VEGF, and E-cadherin in eESCs were assessed as described in Methods. n = 3 per group. *P < 0.05 vs. si-IGF2BP1+ +oe-NC group.

    Techniques Used: EdU Assay, Migration, Transwell Assay, Flow Cytometry, Expressing



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    Image Search Results


    Differential expression of key genes in ectopic endometrial cells. qRT‐PCR validation of four key genes (HOXA10, ESR1, MMP9, and SPP1) in normal endometrial stromal cells (NESCs) and ectopic endometrial stromal cells (EESCs). Gene expression was normalized to GAPDH and presented as fold change relative to NESCs. Data are shown as mean ± SD ( n = 3). ∗∗∗ p < 0.001 versus NESCs.

    Journal: Human Mutation

    Article Title: Single‐Cell Transcriptomic Profiling and Machine Learning Integration Unveil Stromal Cell Heterogeneity in Endometriosis

    doi: 10.1155/humu/5565366

    Figure Lengend Snippet: Differential expression of key genes in ectopic endometrial cells. qRT‐PCR validation of four key genes (HOXA10, ESR1, MMP9, and SPP1) in normal endometrial stromal cells (NESCs) and ectopic endometrial stromal cells (EESCs). Gene expression was normalized to GAPDH and presented as fold change relative to NESCs. Data are shown as mean ± SD ( n = 3). ∗∗∗ p < 0.001 versus NESCs.

    Article Snippet: NESCs (ATCC Cat# CRL‐4003, RRID:CVCL_D697) and EESCs (ATCC Cat# CRL‐7566, RRID:CVCL_IW41) were authenticated by short tandem repeat (STR) profiling, showing ≥ 95% match to the reference profile in the ATCC database.

    Techniques: Quantitative Proteomics, Quantitative RT-PCR, Biomarker Discovery, Gene Expression

    Figure 3. The effects of IGF2BP1 silencing on eESCs. A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The cell viability of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.

    Journal: Folia histochemica et cytobiologica

    Article Title: Inhibition of IGF2BP1 attenuates the progression of endometriosis through PTBP1.

    doi: 10.5603/fhc.98213

    Figure Lengend Snippet: Figure 3. The effects of IGF2BP1 silencing on eESCs. A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The cell viability of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.

    Article Snippet: To assess the viability of eESCs, a cell counting kit-8 (CCK-8, NU679, Dojindo, Kumamoto, Japan) assay was conducted. eESCs were seeded at 5 × 103/well in 96-well plates.

    Techniques: Inverted Microscopy, Flow Cytometry, Migration

    Figure 4. The effects of IGF2BP1/PTBP1 on eESCs. A. The mRNA levels of IGF2BP1 and PTBP1 in eESCs. B. The cell viability of eESCs was evaluated. C. The proliferation was detected using an EDU assay. D. eESC migration and invasion were detected using Transwell assay. E. Flow cytometry was conducted to identify the apoptotic eESC population. F. The expression levels of PCNA, VEGF, and E-cadherin in eESCs were assessed as described in Methods. n = 3 per group. *P < 0.05 vs. si-IGF2BP1+ +oe-NC group.

    Journal: Folia histochemica et cytobiologica

    Article Title: Inhibition of IGF2BP1 attenuates the progression of endometriosis through PTBP1.

    doi: 10.5603/fhc.98213

    Figure Lengend Snippet: Figure 4. The effects of IGF2BP1/PTBP1 on eESCs. A. The mRNA levels of IGF2BP1 and PTBP1 in eESCs. B. The cell viability of eESCs was evaluated. C. The proliferation was detected using an EDU assay. D. eESC migration and invasion were detected using Transwell assay. E. Flow cytometry was conducted to identify the apoptotic eESC population. F. The expression levels of PCNA, VEGF, and E-cadherin in eESCs were assessed as described in Methods. n = 3 per group. *P < 0.05 vs. si-IGF2BP1+ +oe-NC group.

    Article Snippet: To assess the viability of eESCs, a cell counting kit-8 (CCK-8, NU679, Dojindo, Kumamoto, Japan) assay was conducted. eESCs were seeded at 5 × 103/well in 96-well plates.

    Techniques: EdU Assay, Migration, Transwell Assay, Flow Cytometry, Expressing